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Figure 2.

Isolating duplications by trapping. This assay identifies and selectively maintains a preexisting duplication that formed under nonselective conditions. Frequency is defined as the fraction of recombinants that maintain two normally mutually exclusive markers. In this diagram, a Kan recipient receives Tet by a genetic cross. The duplication frequency in the recipient strain is the ratio of Tet Kan duplication transfomants to parental haploid Tet, Kan types. No amplification beyond duplication is selected. Using “recombineering” methods ( Sawitzke et al. 2007 ), the cross that detects the duplication can be performed in recombination-deficient strains.

Trapping was first done by transduction crosses between two closely linked, complementary deletions within the his operon of Salmonella ( Anderson et al. 1976 ). Most His + recombinants from these crosses carry a duplication with one deletion allele in each copy. Because recombination between deletions is rare and preexisting duplications are common, a His + phenotype is most often generated by acquiring the donor allele in one copy of a large preexisting duplication. The donor deletion allele replaces one copy of the recipient allele, generating a heterozygote whose His + phenotype reflects complementation between deletions. In a later experiment, a wild-type his + strain was crossed with a his::Tn10 (His Tet R ) auxotroph and duplication frequency in the recipient was indicated by the fraction of transductants that retained both alleles His + , Tet R ; all such recombinations carried a duplication. Because these assays involve a transductional cross, they could detect duplications only in recombination-proficient cells and could not initially be used to test effects of recombination deficiencies on duplication formation. However, with the advent of recombineering ( FOOTWEAR Ankle boots Baldinini Best Price p9gOYh
), the second marker could be introduced without RecA and the frequency of trapped duplications could be measured in recombination-deficient strains. The frequency of duplication-bearing cells (measured by trapping) was used to estimate the initial rates of duplication formation by determining the frequency attained by a population initiated by a single cell lacking a duplication and grown for a fixed small number of cell generations ( Reams et al. 2010 , 2014 ).

The trapping assay detects cells with two copies of the target site and places no restriction on the size of the amplified region (distance between a and b in Cynthia Cutout Stretchcrepe Midi Dress Black Elizabeth amp; James Shop For Sale Online Perfect For Sale Cheap Best Store To Get Outlet View 6DoIYgK
), as long as that region includes the target. There is no bias toward higher amplification because a single copy of each alternate allele provides full ability to grow under selection. In determining the dependency of duplication rates on various recombination functions, the trapping assays have the advantage of being unaffected by growth rate or viability of the tested recombination mutant. That is, the slower growth and reduced viability of a recA mutant is not expected to reduce the measured duplication rate, which is based on the relative frequency of cells with and without a duplication. Both populations are subject to the same general growth and viability effects, even though the duplication rate may be strongly affected.

Static storage duration variables with constant initializers avoid hard-to-find bugs caused by the indeterminate order of dynamic initialization. They can also be safely used during dynamic initialization across translation units.

This attribute acts as a compile time assertion that the requirements for constant initialization have been met. Since these requirements change between dialects and have subtle pitfalls it’s important to fail fast instead of silently falling back on dynamic initialization.

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The attribute allows you to specify a specific section a global variable or function should be in after translation.

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The attribute marks a parameter of a function as having the special context-parameter ABI treatment.

This treatment generally passes the context value in a special register which is normally callee-preserved.

A parameter must either be the last parameter or must be followed by a parameter (which itself must always be the last parameter).

A context parameter must have pointer or reference type.

swift_error_result (clang::swift_error_result, clang::swift_error_result)

The attribute marks a parameter of a function as having the special error-result ABI treatment.

This treatment generally passes the underlying error value in and out of the function through a special register which is normally callee-preserved. This is modeled in C by pretending that the register is addressable memory:

A parameter must be the last parameter, and it must be preceded by a parameter.

A parameter must have type or for some type T. Note that no qualifiers are permitted on the intermediate level.

It is undefined behavior if the caller does not pass a pointer or reference to a valid object.

The standard convention is that the error value itself (that is, the value stored in the apparent argument) will be null upon function entry, but this is not enforced by the ABI.

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The attribute marks a parameter of a function as having the special indirect-result ABI treatment.

This treatment gives the parameter the target’s normal indirect-result ABI treatment, which may involve passing it differently from an ordinary parameter. However, only the first indirect result will receive this treatment. Furthermore, low-level lowering may decide that a direct result must be returned indirectly; if so, this will take priority over the parameters.

A parameter must either be the first parameter or follow another parameter.

A parameter must have type or for some object type . If is a complete type at the point of definition of a function, it is undefined behavior if the argument value does not point to storage of adequate size and alignment for a value of type .

Making indirect results explicit in the signature allows C functions to directly construct objects into them without relying on language optimizations like C++’s named return value optimization (NRVO).

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The attribute indicates that a function should be called using the Swift calling convention for a function or function pointer.

The lowering for the Swift calling convention, as described by the Swift ABI documentation, occurs in multiple phases. The first, “high-level” phase breaks down the formal parameters and results into innately direct and indirect components, adds implicit paraameters for the generic signature, and assigns the context and error ABI treatments to parameters where applicable. The second phase breaks down the direct parameters and results from the first phase and assigns them to registers or the stack. The convention only handles this second phase of lowering; the C function type must accurately reflect the results of the first phase, as follows:

does not support variadic arguments or unprototyped functions.

The parameter ABI treatment attributes are aspects of the function type. A function type which which applies an ABI treatment attribute to a parameter is a different type from an otherwise-identical function type that does not. A single parameter may not have multiple ABI treatment attributes.

Support for this feature is target-dependent, although it should be supported on every target that Swift supports. Query for this support with . This implies support for the , , and attributes.

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The attribute declares a variable with thread local storage. It is available under the flag for MSVC compatibility. See the documentation for __declspec(thread) on MSDN.

In Clang, is generally equivalent in functionality to the GNU keyword. The variable must not have a destructor and must have a constant initializer, if any. The attribute only applies to variables declared with static storage duration, such as globals, class static data members, and static locals.

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The attribute allows you to specify which thread-local storage model to use. It accepts the following strings:

TLS models are mutually exclusive.

The trivial_abi attribute can be applied to a C++ class, struct, or union. It instructs the compiler to pass and return the type using the C ABI for the underlying type when the type would otherwise be considered non-trivial for the purpose of calls. A class annotated with trivial_abi can have non-trivial destructors or copy/move constructors without automatically becoming non-trivial for the purposes of calls. For example:

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